Patent Blog
  • Contribute
  • Recommend
  • Subscribe
  • Login
  • Our Services

TODAY’S PATENT – METHODS OF AMPLIFYING NUCLEIC ACID SEQUENCES MEDIATED BY TRANSPOSASE/TRANSPOSON DNA COMPLEXES

  • Older
  • Newer

TODAY’S PATENT – METHODS OF AMPLIFYING NUCLEIC ACID SEQUENCES MEDIATED BY TRANSPOSASE/TRANSPOSON DNA COMPLEXES

Today’s patent was invented by Chongyi Chen, Dong Xing, and Xiaoliang Sunney Xie and was granted patent on January 19, 2012, bearing patent no. US10894980.

Methods are provided for nucleic acid amplification including contacting a double stranded nucleic acid with transposases bound to transposon DNA, wherein the transposon DNA includes a transposase binding site and an RNA polymerase promoter sequence, wherein the transposases/transposon DNA complex bind to target locations along the double stranded nucleic acid and cleave the double stranded nucleic acid into a plurality of double stranded fragments, with each double stranded fragment having the transposon DNA bound to each 5′ end of the double stranded fragment, extending the double stranded fragments along the transposon DNA to make double stranded extension products having double stranded RNA polymerase promoter sequences at each end, contacting the double stranded extension products with an RNA polymerase to make a plurality of RNA transcripts of each double stranded extension product, reverse transcribing the RNA transcripts into single stranded copy DNA, forming complementary strands to the single stranded copy DNA to form a plurality of double stranded DNA amplicons corresponding to each double stranded fragment.

  • Categories
    Today's Patent
  • Author

    Tanya Janghel

Leave a Reply Cancel Reply

Your email address will not be published. Required fields are marked *

Related Posts

TODAY’S PATENT – MULTIFUNCTIONAL IMPLEMENT FOR FARMING AND GARDENING

TODAY’S PATENT – CHIMNEY CLEANING APPARATUS

TODAY’S PATENT – BODY SHOWER

Lex Protector

International Law Office

🇺🇸 +1 888 890 6411

Ip Enforcement

  • Copyright Enforcement
  • Utility Patent Enforcement
  • Design Patent Enforcement
  • Trademark Enforcement

Copyright Registration

  • USA Copyright Registration
  • Indian Copyright Registration
  • Amazon Brand Registration

Subscribe

© Copyright 2020. All rights Reserved

  • Terms & Conditions
  • Privacy Policy

Contribute

Recommend

    Subscribe