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TODAY’S PATENT – REVERSE TWO-HYBRID SYSTEM

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TODAY’S PATENT – REVERSE TWO-HYBRID SYSTEM

The REVERSE TWO-HYBRID SYSTEM was invented by Marc Vidal, Jef D. Boeke and Ed Harlow on the 21st of September 1999, bearing patent number US5955280A.

This present invention generally relates to methods for identifying molecular interactions (e.g., protein/protein, protein/DNA, protein/RNA, or RNA/RNA interactions). All of the methods within the invention employ counterselection and at least two hybrid molecules. Molecules which interact reconstitute a transcription factor and direct expression of a reporter gene, the expression of which is then assayed. Also disclosed are genetic constructs which are useful in practicing the methods of the invention.

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    Satyakam Baliarsingh

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