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TODAY’S PATENT – DNA DIAGNOSTICS BASED ON MASS SPECTROMETRY

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TODAY’S PATENT – DNA DIAGNOSTICS BASED ON MASS SPECTROMETRY

The DNA diagnostics based on mass spectrometry was invented by Hubert KösterDaniel P. LittleAndreas Braun on the 5th August, 2003, bearing patent number US6602662B1.

Fast and highly accurate mass spectrometry-based processes for detecting particular nucleic acid molecules and sequences in the molecules are provided. Depending upon the sequence to be detected, the processes, for example, can be used to diagnose a genetic disease or a chromosomal abnormality, a predisposition to a disease or condition, or infection by a pathogen, or for determining identity or heredity. One aspect provides a process for determining whether a target nucleotide is present in a nucleic acid molecule including hybridizing a nucleic acid molecule with a primer oligonucleotide; contacting the hybridized primer with deoxyribonucleoside triphosphates, chain terminating nucleotides and a DNA polymerase, whereby the hybridized primer is extended until a chain terminating nucleotide is incorporated, producing an extended primer, and determining the molecular mass of the extended primer, thereby determining whether the target nucleotide is present in a nucleic acid molecule.

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    Ishita Goyal

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